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1.
A contraction of: it was not
'Twasn't the night before christmas.
door The-Whale-Bullier 17 april 2011
 
2.
The Old English new age combination of the words: It, Was, and Not.
"What night did that burglar break into the house?"

"T'wasn't the night before Christmas."

"You're a douche."
door Benjiforce1 30 september 2010
 
3.
the area on a man between the nutsack and the asshole
if twasnt there - your insides would fall out
door Beaver660 5 augustus 2006
 
4.
Its an alternate form of saying the word "Taint."
She tickled the area near my undercarriage that twasnt my balls or ass.
door Steve 13 december 2003